Intermediate
Part:BBa_K1510928:Design
Designed by: Kao Jung Chang Group: iGEM14_NYMU-Taipei (2014-10-07)
RFP indicated INPNC performance evaluation
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1036
Illegal AgeI site found at 2212
Illegal AgeI site found at 2324 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We consider the E1010 performance in the aspect of its efficiency and its DNA size, in afraid of giving gene express too much burden.
Source
We truncated INPNC ourselves, derive C16 from Streptococcus Mutants and we ligate an igem BBa_E1010 RFP at the tail of this surface display gene.